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type i procollagen levels  (R&D Systems)


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    Structured Review

    R&D Systems type i procollagen levels
    Type I Procollagen Levels, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 129 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/type+i+procollagen+levels/Human+Pro-Collagen+I+alpha+1+DuoSet+ELISA/pm41351364-49-43-55
    Average 96 stars, based on 129 article reviews
    type i procollagen levels - by Bioz Stars, 2026-09
    96/100 stars

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    Incubation:

    Article Title: Skin regenerative potential of polydeoxyribonucleotide isolated from Saussurea involucrata.
    Article Snippet: The wound area was viewed at 0 and 24 h using a light microscope (Leica Microsystems, Wetzlar, Germany), and wound width was quantified using ImageJ software. .. HDFs (2 × 104 cells/well) were grown in 24-well plates (SPL) and incubated for 24 h. The cells were then treated with snow lotus PDRN at various concentrations (1, 5, or 10 μg/mL) for 72 h. Culture supernatants were collected and quantified for type I procollagen levels using a human type I procollagen ELISA kit (R&D Systems, Minneapolis, MN, USA) following the manufacturer’s instructions. ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Skin regenerative potential of polydeoxyribonucleotide isolated from Saussurea involucrata.
    Article Snippet: The wound area was viewed at 0 and 24 h using a light microscope (Leica Microsystems, Wetzlar, Germany), and wound width was quantified using ImageJ software. .. HDFs (2 × 104 cells/well) were grown in 24-well plates (SPL) and incubated for 24 h. The cells were then treated with snow lotus PDRN at various concentrations (1, 5, or 10 μg/mL) for 72 h. Culture supernatants were collected and quantified for type I procollagen levels using a human type I procollagen ELISA kit (R&D Systems, Minneapolis, MN, USA) following the manufacturer’s instructions. ..



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    Fig. 5. Procollagen type I C-peptide <t>(PIP)</t> <t>levels</t> in HDF culture medium supernatants 24 hr after treatment with glycoside-rich C. asiatica extracts (GRCs) at various concentrations (0.1, 1, and 10 µg/ml). *p < 0.05 vs. 1 % DMSO (control).
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    Image Search Results


    Effects on serum (A) 25(OH)D, (B) 1,25(OH) 2 D, (C) ALP, (D) Ca + , (E) OCN, (F) PINP concentration in mice. * indicates significant differences from the model group: * p < 0.05, ** p < 0.01, and *** p < 0.001.

    Journal: Frontiers in Nutrition

    Article Title: Bifidobacterium adolescentis CCFM1447 effectively alleviates osteoporosis by enriching intestinal flora capable of vitamin D conversion

    doi: 10.3389/fnut.2025.1647671

    Figure Lengend Snippet: Effects on serum (A) 25(OH)D, (B) 1,25(OH) 2 D, (C) ALP, (D) Ca + , (E) OCN, (F) PINP concentration in mice. * indicates significant differences from the model group: * p < 0.05, ** p < 0.01, and *** p < 0.001.

    Article Snippet: Osteocalcin (OCN) and Procollagen type I N-propeptide (PINP) levels were measured using commercial Mouse ELISA kits (E-EL-M0864, E-EL-M0233, Elabscience Biotechnology).

    Techniques: Concentration Assay

    (A) Levels of 1,25(OH) 2 D in the supernatants of key differential bacteria after separate cultures; Effects of key differential flora on serum (B) 25(OH)D, (C) 1,25(OH) 2 D, (D) OCN, (E) PINP concentration in mice. * indicates significant differences from the model group: * p < 0.05, ** p < 0.01, and *** p < 0.001.

    Journal: Frontiers in Nutrition

    Article Title: Bifidobacterium adolescentis CCFM1447 effectively alleviates osteoporosis by enriching intestinal flora capable of vitamin D conversion

    doi: 10.3389/fnut.2025.1647671

    Figure Lengend Snippet: (A) Levels of 1,25(OH) 2 D in the supernatants of key differential bacteria after separate cultures; Effects of key differential flora on serum (B) 25(OH)D, (C) 1,25(OH) 2 D, (D) OCN, (E) PINP concentration in mice. * indicates significant differences from the model group: * p < 0.05, ** p < 0.01, and *** p < 0.001.

    Article Snippet: Osteocalcin (OCN) and Procollagen type I N-propeptide (PINP) levels were measured using commercial Mouse ELISA kits (E-EL-M0864, E-EL-M0233, Elabscience Biotechnology).

    Techniques: Bacteria, Concentration Assay

    Fig. 5. Procollagen type I C-peptide (PIP) levels in HDF culture medium supernatants 24 hr after treatment with glycoside-rich C. asiatica extracts (GRCs) at various concentrations (0.1, 1, and 10 µg/ml). *p < 0.05 vs. 1 % DMSO (control).

    Journal: Phytomedicine Plus

    Article Title: The effects of glycoside-rich green extract from Centella asiatica (L.) Urban on wound healing and anti-aging activity

    doi: 10.1016/j.phyplu.2024.100628

    Figure Lengend Snippet: Fig. 5. Procollagen type I C-peptide (PIP) levels in HDF culture medium supernatants 24 hr after treatment with glycoside-rich C. asiatica extracts (GRCs) at various concentrations (0.1, 1, and 10 µg/ml). *p < 0.05 vs. 1 % DMSO (control).

    Article Snippet: After 24 hr of incubation, the culture supernatants were collected, and the PIP levels were determined by following the PIP ELISA Kit protocol (MK101, Takara Bio, Inc.).

    Techniques: Control

    Fig. 6. Procollagen type I C-peptide (PIP) levels in HDF culture medium supernatants 24 hr after treatment with 10 µg/ml GRC1 and different components: total triterpenoids (MS+AS+MA+AA), glycosides (MS+AS), aglycones (MA+AA) and individual triterpenoids (MS, AS, MA, AA). *p < 0.05 vs. 1 % DMSO (control) #p < 0.05 vs. 10 µg/ml GRC1 MS=Madecassoside; AS=Asiaticoside; MA=Madecassic acid; AA=Asiatic acid.

    Journal: Phytomedicine Plus

    Article Title: The effects of glycoside-rich green extract from Centella asiatica (L.) Urban on wound healing and anti-aging activity

    doi: 10.1016/j.phyplu.2024.100628

    Figure Lengend Snippet: Fig. 6. Procollagen type I C-peptide (PIP) levels in HDF culture medium supernatants 24 hr after treatment with 10 µg/ml GRC1 and different components: total triterpenoids (MS+AS+MA+AA), glycosides (MS+AS), aglycones (MA+AA) and individual triterpenoids (MS, AS, MA, AA). *p < 0.05 vs. 1 % DMSO (control) #p < 0.05 vs. 10 µg/ml GRC1 MS=Madecassoside; AS=Asiaticoside; MA=Madecassic acid; AA=Asiatic acid.

    Article Snippet: After 24 hr of incubation, the culture supernatants were collected, and the PIP levels were determined by following the PIP ELISA Kit protocol (MK101, Takara Bio, Inc.).

    Techniques: Control